Uncovering phenotypic inheritance from single cells with Microcolony-seq
We developed Microcolony-seq for determining phenotypic and genetic heterogeneity in bacterial cultures. Appling the method directly to human urine and bloodstream infections, we find phenotypically different bacterial states, differing at their virulence and stress responses. These hidden bacterial states can shape disease outcomes.
Formation of a membraneless compartment regulates bacterial virulence
We show that CsrA forms a dynamic membraneless compartment in pathogenic bacteria, which also contains degradosome components, regulatory sRNAs, and target mRNAs, and that its formation is linked to a switch in CsrA’s regulatory effect on virulence gene expression.
Mapping the small RNA interactome in bacteria using
RIL-seq
We include the full protocol for the RIL-seq methodology for transcriptome-wide mapping of bacterial sRNA-target pairs that captures in vivo interactions without overexpression of sRNAs. By co-immunoprecipitating Hfq-bound RNAs followed by a computational pipeline, RIL-seq identifies significant sRNA–target interactions, enabling the study of the dynamic post-transcriptional regulatory network.
Global Mapping of Small RNA-Target Interactions in Bacteria
We developed a broadly applicable methodology termed RIL-seq (RNA interaction by ligation and sequencing), which integrates experimental and computational tools for in vivo transcriptome-wide identification of interactions involving Hfq-associated sRNAs. By applying this methodology to Escherichia coli we discovered an extensive network of interactions involving RNA pairs showing sequence complementarity.
Growth of poorly differentiated endometrial carcinoma is inhibited by combined action of medroxyprogesterone acetate and the Ras inhibitor Salirasib
We developed a novel drug combination of progestin medroxyprogesterone acetate (MPA) and Ras inhibitor S-farnesylthiosalicylic acid (FTS) to treat aggressive, hormone-resistant type 2 endometrial carcinoma (EC). The combination reduced tumor cell proliferation, enhanced cell death, and inhibited ERα-mediated gene expression, offering a potential new treatment for this refractory cancer.
Bromine- and Chlorine-Containing Aeruginosins from Microcystis aeruginosa Bloom Material Collected in Kibbutz Geva, Israel
We identified and characterized five new aeruginosin peptides from a Microcystis aeruginosa bloom in Israel, alongside four known variants. Aeruginosins are protease inhibitors produced by cyanobacteria, and their structural diversity suggests potential for drug discovery and understanding cyanobacterial chemical ecology.